Syringe Purification and HRP-Conjugation of Goat IgGs Used in Quality Control of Erythropoietin

Marcel Bassil, Nataliia Pavliuchenko, Elia Raya

Abstract


Goat anti-rabbit IgGs were purified from serum using Protein G affinity chromatography. The purity of eluted fractions was tested using SDS-PAGE, and the functionality of IgGs was tested in Western Blot analysis of Erythropoietin, after conjugation with Horseradish Peroxidase.


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DOI: https://doi.org/10.5296/jab.v6i2.12829

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