The Immunomodulation of Mangiferin on the Proliferation and Interleukin-2 Receptors Expression of in Vitro Culture of T lymphocytes Derived from Mammary Tumor Bearing Mice

Supri Irianti Handayani, . Kusmardi, Aryo Tedjo

Abstract


The development of a tumor has a strong relationship with cellular immunity mediated by T lymphocytes. Some research showed that mangiferin can increase host immunity but its scientific base was still unclear. The purpose of this study was to explain the role of mangiferin as an immunostimulator on in vitro T lymphocytes culture of mammary tumor bearing mice. T lymphocytes were isolated from the spleen of mice after two weeks of mammary tumor transplantation. The isolation was performed by using sterile nylon net and passed through nylon wool. Isolated T lymphocytes were cultured in vitro on RPMI 1640 medium. The culture of T lymphocytes was divided into three groups containing 5, 25 and 50 mg/L of mangiferin, and a control group (RPMI medium). The proportion of T lymphocytes and IL-2Rα (CD25) expressed T cells were evaluated using immunoflouorescens techniques after one and three days of lymphocytes cultures. Our study showed that 5, 25 and 50 mg/L mangiferin was effective to enhance IL-2Rα (CD25) on T lymphocytes culture and T lymphocytes prolifferation.


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References


Agarwala, S., Rao, B. N., Mudholkar, K., Bhuwania, R., & Rao, B. S. (2012). Mangiferin, a Dietary Xanthone Protects Against Mercury-Induced Toxicity in HepG2 Cells. Environ Toxicol, 27(2), 117-127. http://dx.doi.org/10.1002/tox.20620

Chattopadhyay, U., Das, S., Guha, S., Ghosal, S. (1987). Activation of lymphocytes of normal and tumor bearing mice by mangiferin, a naturally occurring glucosylxanthone. Cancer Lett., 37(3), 293-9. http://dx.doi.org/10.1016/0304-3835(87)90114-5

Curigliano, G. (2011). Immunity and autoimmunity: revising the concepts of response to breast cancer. Breas, 20(supp 3), S71-S74. http://dx.doi.org/10.1016/S0960-9776(11)70298-3

de Visser, K. E., Eichten, A., & Coussens, L. M. (2006). Paradoxical roles of the immune system during cancer development. Nat Rev Cancer, 6(1), 24-37. http://dx.doi.org/10.1038/nrc1782

García, D., Delgado, R., Ubeira, F. M., & Leiro, J. (2002). Modulation of rat macrophage function by the Mangifera indica L. extracts Vimang and mangiferin. Int Immunopharmacol, 2(6), 797-806. http://dx.doi.org/10.1016/S1567-5769(02)00018-8

García, D., Leiro, J., Delgado, R., Sanmartín, M. L., & Ubeira, F. M. (2003). Mangifera indica L. extract (Vimang) and mangiferin modulate mouse humoral immuneresponses. Phytother Res., 17(10), 1182-7. http://dx.doi.org/10.1002/ptr.1338

Garrido, G., Delgado, R., Lemus, Y., Rodríguez, J., García, D., & Núñez-Sellés, A. J. (2004a). Protection against septic shock and suppression of tumor necrosis factor alpha and nitric oxide production on macrophages and microglia by a standard aqueous extract of Mangifera indica L. (VIMANG). Role of mangiferin isolated from the extract. Pharmacol Res, 50(2), 165-72. http://dx.doi.org/10.1016/j.phrs.2003.12.020

Garrido, G., González, D., Lemus, Y., García, D., Lodeiro, L., Quintero, G., … Delgado, R. (2004b). In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG). Pharmacol Res., 50(2), 143-9. http://dx.doi.org/10.1016/j.phrs.2003.12.003

Gooden, M. J. M., de Bock, G. H., Leffers, N., Daemen, T., & Nijman, H. W. (2011). The prognostic influence of tumour-infiltrating lymphocytes in cancer: a systematic review with meta-analysis. Br J Cancer, 105(1), 93-103. http://dx.doi.org/10.1038%2Fbjc.2011.189

Grivennikov, S. I., Greten, F. R., & Karin, M. (2010). Immunity, inflammation, and cancer. Cell, 140(6), 883-899. http://dx.doi.org/10.1016%2Fj.cell.2010.01.025

Hernandez, P., Rodriguez, P. C., Delgado, R., & Walczak, H. (2007). Protective effect of Mangifera indica L. polyphenols on human T lymphocytes against activation-induced cell death. Pharmacol Res., 55(2), 167-73. http://dx.doi.org/10.1016/j.phrs.2006.11.004

Macchetti, A. H., Marana, H. R. C., Silva, J. S., de Andrade, J. M., Ribeiro-Silva, A., & Bighetti, S. (2006). Tumor-infiltrating CD4+ T lymphocytes in early breast cancer reflect lymph node involvement. Clinics, 61(3), 203-208. http://dx.doi.org/10.1590/S1807-59322006000300004

Morgan, S. J., & Darling, D. C. (1993). Animal Cell Culture (pp. 27-36). United Kingdom: Bios Scintific Publisher Limited.

Muruganandan, S., Lal, J., & Gupta, P. K. (2005a). Immunotherapeutic effects of mangiferin mediated by the inhibition of oxidative stress to activated lymphocytes, neutrophils and macrophages. Toxicol., 215(1-2), 57-68. http://dx.doi.org/10.1016/j.tox.2005.06.008

Muruganandan, S., Srinivasan, S. K., Gupta, P. K., & Gupta, J. L. (2005b). Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. J Ethnopharmacol, 97, 497-501. http://dx.doi.org/10.1016/j.jep.2004.12.010

Sánchez, G. M., Re, L., Giuliani, A., Núñez-Sellés, A. J., Davison, G. P., & León-Fernández, O. S. (2000). Protective effects of Mangifera indica L. extract, mangiferin and selected antioxidants against TPA-induced biomolecules oxidation and peritoneal macrophage activation in mice. Pharmacol Res., 42(6), 565-73. http://dx.doi.org/10.1006/phrs.2000.0727

Sheu, B., Kuo, W., Chen, R., Huang, S., Chang, K., & Chow, S. (2008). Clinical significance of tumor-infiltrating lymphocytes in neoplastic progression and lymph node metastasis of human breast cancer. Breast., 17(6), 604-610. http://dx.doi.org/10.1016/j.breast.2008.06.001

Viswanadh, E. K., Rao, B. N., & Rao, B. S. (2010). Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride Hum Exp Toxicol., 29, 409-418. http://dx.doi.org/10.1177/0960327110361752

Prestwich, R. J., Errington, F., & Hatfield, P. (2008). The immune system: is it relevant to cancer development, progression and treatment? Clin Oncol., 20(2), 101-112. http://dx.doi.org/10.1016/j.clon.2007.10.011

Vesely, M. D., Kershaw, M. H., Schreiber, R. D., & Smyth, M. J. (2011). Natural innate and adaptive immunity to cancer. Ann Rev Immunol, 29, 235-271. http://dx.doi.org/10.1146/annurev-immunol-031210-101324




DOI: https://doi.org/10.5296/jab.v3i2.6423

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