Immunohistochemistry of Heat Shock Protein 70 (Hsp70) on Earthworm (Lumbricus terrestris) in Gold Mining Sites

Abstract

The traditional gold mining in Cempaka, Banjarbaru, South Kalimantan, Indonesia used mercury in order to separate the gold with a fine sand and rocks. Mercury can impair the function of the cellular and organism physiology that live in the mining sites. In cellular on earthworm may adapt to help in repairing cell damage with expression of HSP70 proteins. This study was conducted in November 2012 to September 2014. Sampling of soil and earthworms do in Banjarbaru village (control) and in paddy field near gold mining among them Murung village, Pumpung village and Palam village (ex. PT. Galuh Cempaka). Soil quality in the gold mining indicate conditions are still good in term of C-organic, pH and CEC. The C-organic content of the soil between the 2.08-2.52%; the value of pH H₂O between 4.90-5.72, CEC between 17.20-18.11. Detection of protein HSP70 on earthworms use the method immunohistochemistry. HSP70 expression was found in the longitudinal muscle layer (LM). The expression of HSP70 most occurred in earthworms that live in the land of the paddy fields ex. PT. Galuh Cempaka.