The Immunomodulation of Mangiferin on the Proliferation and Interleukin-2 Receptors Expression of in Vitro Culture of T lymphocytes Derived from Mammary Tumor Bearing Mice

Supri Irianti Handayani, . Kusmardi, Aryo Tedjo


The development of a tumor has a strong relationship with cellular immunity mediated by T lymphocytes. Some research showed that mangiferin can increase host immunity but its scientific base was still unclear. The purpose of this study was to explain the role of mangiferin as an immunostimulator on in vitro T lymphocytes culture of mammary tumor bearing mice. T lymphocytes were isolated from the spleen of mice after two weeks of mammary tumor transplantation. The isolation was performed by using sterile nylon net and passed through nylon wool. Isolated T lymphocytes were cultured in vitro on RPMI 1640 medium. The culture of T lymphocytes was divided into three groups containing 5, 25 and 50 mg/L of mangiferin, and a control group (RPMI medium). The proportion of T lymphocytes and IL-2Rα (CD25) expressed T cells were evaluated using immunoflouorescens techniques after one and three days of lymphocytes cultures. Our study showed that 5, 25 and 50 mg/L mangiferin was effective to enhance IL-2Rα (CD25) on T lymphocytes culture and T lymphocytes prolifferation.

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