Partial purification and biochemical characterization of amylase from Aeromonas caviaeNK1isolated from Industrial waste of India

Kalyani Khanra, Indranil Choudhuri, Sudipta Panja, Nandan Bhattacharyya

Abstract


A partial purification and biochemical characterization of theamylase from AeromonascaviaeNK1 were carried out in this study. The extracellular extract was concentrated using ammonium sulfate precipitation and optimum operational conditions for the enzyme activity from the strain were evaluated. The optimum pH and temperature were observed 11.5 and370C respectively. Ca2+, Mn2+, Mg2+, Zn2+, Pb2+, Co2+ were found to have effect on amylase activity.Furthermore, the analysis of kinetic showed that the enzyme has ๐พ๐‘š of 2.4mg/mL and ๐‘‰max of 21853.0๐œ‡mol/min/mg for starch. The results indicate that the enzyme reflects their potentiality towards industrial utilization.


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DOI: https://doi.org/10.5296/jbls.v7i1.8361

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