The Effect of Rosemary (Rosmarinus officinalis. L) Plant Extracts on the Immune Response and Lipid Profile in Mice

Fawzi Mohammad Al Sheyab, Nizar Abuharfeil, Lina Salloum, Rehan Bani Hani, Dalal Saleh Awad

Abstract


The hypolipidemic activity of leaves of rosemary (Rosmarinus officinalis), a herbal plant was studied. Three groups of BALB/c mice were treated as following: the first group was fed with normal diet (sugar & water), the second group was fed with high cholesterol (HC) (2% by weight) and coconut oil (25% by weight), for 36 days, and the third group was fed with HC diet and given 100 mg/kg rosemary extract (10% w/v) during the last 15 days of treatment period. There was a significant decrease (P < 0.001) in plasma total cholesterol (TC)(- 68.57%), low density lipoprotein (LDL) (- 56.34%), and triglycerides (TG) (- 182.61%). A significant increase in high density lipoprotein (HDL) (38.53%) was obtained in rosemary-fed mice compared to HC mice. In another experiment, the immunomodulatory activity of aqueous extract of R. officinalis was evaluated in BALB/c mice. Mice were treated with three doses of extract (10, 50, 100 mg/kg body weight) for 8 weeks. Humoral immunity against membrane proteins of sheep erythrocytes measured by ELISA showed that IgM (Immunoglobulin M) response significantly increased by 26.95%, 36.5%, 70.78% respectively, in mice fed with 10, 50, and 100 mg/kg of rosemary as compared to the control group (P < 0.05). IgG (Immunoglobulin G) response increased significantly at all antibody titers, in mice fed with rosemary at doses of 10, 50, and 100 mg/kg. Meanwhile, there was no significant different in IgM and IgG responses between 50 and 100 mg/kg (P > 0.05). On the other hand, concanavalin A-stimulated proliferation of spleen cells from mice fed with 100 mg rosemary extract was significantly higher by 57% than that of cells from the corresponding control animals.


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DOI: http://dx.doi.org/10.5296/jbls.v3i1.906

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Journal of Biology and Life Science  ISSN 2157-6076

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