Isolation, Identification and Molecular Characterization of Nocardiopsaceae Producing Novel Antibiotics From Soil Samples in Bingol, Turkey

Samer Abuzerr, Kate Zinszer, Syamand Ahmed Qadir, Ekrem Atalan, Halgord Ali M. Farag, Abdel Hamid el Bilbeisi, Kawa Khwarahm Hamafaraj, Rima Naser, Miran Husein Qader, Alshaarawi Salem, Amany Al Afifi, Salem Abu Musleh

Abstract


There is growing interest in the antimicrobial discovery of life-threatening multidrug-resistant pathogens. The study was undertaken to isolate, identify, and characterize antibiotic-producing actinomycetaceae, particularly nocadisosaceae, from soil samples of Bingol, Turkey. Soil samples were collected from three different regions of Bingol, Turkey. The physicochemical analysis of the soil samples was immediately measured using standard methods. This was followed by isolation of Nocardiopsaceae, nutritional tests, chemotaxonomic analysis, and molecular characterization. The isolated organisms showed morphological properties consistent with the Nocardiopsaceae soil bacteria.  The 16s rDNA gene sequence indicated a similarity between the strains with 99.86% which was Nocardiopsaceae synnemata-formans. The BLAST hits had a significant e-value of 0.005. The results of the present study revealed that soil Nocardiopsaceae of Bingol appears to have immense potential as a source of antibacterial compounds.


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DOI: https://doi.org/10.5296/jbls.v11i2.17050

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Copyright (c) 2020 Samer Abuzerr, Kate Zinszer, Syamand Ahmed Qadir, Ekrem Atalan, Halgord Ali M. Farag, Abdel Hamid el Bilbeisi, Kawa Khwarahm Hamafaraj, Rima Naser, Miran Husein Qader, Alshaarawi Salem, Amany Al Afifi, Salem Abu Musleh

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Journal of Biology and Life Science  ISSN 2157-6076

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