Influence of Prebiotics, Probiotics, and Synbiotics on the Hematology and Serum - Biochemical Parameters of Broiler Chickens

Abstract

This study investigated the effects of Prebiotics, Probiotics, and Synbiotics as alternatives to in-feed antimicrobials on the hematological and serum biochemical parameters of broiler chickens. A total of 200 broilers were fed diets supplemented with these alternatives for 42 days. On days 14, 28, and 42, blood samples were collected for parameter analyses. R software, one-way analysis of variance followed by mean comparison using the Least Significant Difference post hoc test were used in data analyses. The results indicated these supplements significantly improved levels of aspartate aminotransferase, alkaline phosphatase, and blood urea nitrogen at different trial periods. The synbiotic-treated group exhibited the highest total protein concentrations and improved hematological profiles, including increased red blood cell count and hematocrit. Although red blood cell counts showed a slight increase across all groups, the differences were not statistically significant. However, hematocrit values were significantly higher in the synbiotic group on day 42 (P = 0.037). Conversely, alanine aminotransferase and creatinine levels did not differ significantly among the treatment groups. Interestingly, white blood cell counts were significantly elevated in the negative control group (P < 0.05), while hemoglobin levels showed a significant increase in the synbiotic group on day 28 (P = 0.042). Overall, the study findings suggest that these antimicrobial alternatives can positively influence physiological health markers in broiler chickens and may serve as viable alternatives to in-feed antimicrobials. Future studies are encouraged to explore the long-term effects of these alternatives.